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For HE staining, right after drying the slides for 30 seconds at place temperature, they ended up put in xylene for one moment, adopted by a hundred%, ninety five%, 80% and 70% ethanol for one moment, respectively. After dipping in distilled h2o for thirty seconds, haematoxylin staining was done for 5 minutes, dipping into dH2O yet again, and then putting the slides in Eosin answer for two minute